Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation.
نویسندگان
چکیده
منابع مشابه
Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation.
AIMS To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment. METHODS Cervical smears and cytological samples were obtained from 122 patients during the same clinical examination and the presence of HPV ...
متن کاملComparative analysis of human papillomavirus detection by PCR and non-isotopic in situ hybridisation.
AIMS--To assess the relative diagnostic performance of the polymerase chain reaction (PCR) and non-isotopic in situ hybridisation (NISH) and to correlate these data with cytopathological assessment. METHODS--Paired analysis of human papillomavirus (HPV) detection was performed by PCR and NISH on exfoliated cervical cells from 122 women attending a routine gynaecological examination. PCR amplifi...
متن کاملDetection of human papillomavirus infection by non-isotopic in situ hybridisation in condylomatous and CIN lesions.
AIMS To study the value of non-isotopic in situ hybridisation (NISH) in detecting human papillomavirus (HPV) infection in female genital lesions positive for the virus by conventional histology but negative by filter DNA hybridisation. METHODS Forty three cases, which showed the histological hallmarks of the HPV infection but produced negative results in filter dot blot hybridisation tests (V...
متن کاملEpisomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation.
It was postulated that non-isotopic in situ hybridisation (NISH) signal types 1-3 for human papillomavirus in cervical biopsy specimens represent episomal or integrated virus. The aim of this study was to validate this hypothesis by independent molecular techniques. Fresh cervical intraepithelial neoplasia (CIN) and squamous cell cancer (SCC) tissue were examined for NISH signal pattern by hybr...
متن کاملDetection of human papillomavirus in matched cervical smears and biopsy specimens by non-isotopic in situ hybridisation.
AIMS To determine the relative diagnostic sensitivity of non-isotopic in situ hybridisation (NISH) for the diagnosis of human papillomavirus (HPV) on matched smears and biopsy specimens; to compare the NISH signal type in the two samples; and to correlate the NISH data with the morphological diagnosis. METHODS HPV samples were assayed individually by NISH with digoxigenin labelled probes (HPV...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1992
ISSN: 0021-9746
DOI: 10.1136/jcp.45.10.866